Apr 06, 2024
Nuclei Isolation for Human Ovary Explants
- Nicolas Martin1
- 1Buck Institute for research on Aging
Protocol Citation: Nicolas Martin 2024. Nuclei Isolation for Human Ovary Explants. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvw1632lmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 05, 2024
Last Modified: April 06, 2024
Protocol Integer ID: 97862
Keywords: nuclei isolation for human ovary explant, fresh frozen human ovary explant, human ovary explant, nuclei isolation, nuclei suspension, nuclei, frozen human ovary explant
Disclaimer
This protocol needs prior approval by the users' institutional review board (IRB) or equivalent ethics committee(s).
Abstract
This protocol uses fresh frozen human ovary explants to isolate nuclei suspension.
Guidelines
This protocol needs prior approval by the users' institutional review board (IRB) or equivalent ethics committee(s).
Materials
Refers to the various protocol documents for a complete list of the material required.
Nuclei Isolation Protocol for Human Ovary Explants
Chapter 1—Single Cell Gene Expression & Chromium Fixed RNA Profiling of the protocol CG000505 REV A was used to isolate nuclei from frozen human ovary explants with the following modifications: 1) a cordless motor pestle (VWR, Catalog number 47747-370) was used for Step f, Page 30 and 2) the samples were incubated for 15 min on ice for Step h, Page 30.
Protocol references
The following protocols from 10X Genomics were used for the different steps:
Nuclei Isolation: CG000505 REV A