Nov 23, 2023

Public workspaceNovel Object Recognition Test

Nature Communications
DOI
https://dx.doi.org/10.17504/protocols.io.81wgbx99qlpk/v1
  • Marina Lorente Picón1,
  • Núria Peñuelas1,
  • Ariadna Laguna1,
  • Miquel Vila1
  • 1Vall d'Hebron Research Institute
  • Vilalab Public
  • Nuria
Miquel Vila
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DOI: https://dx.doi.org/10.17504/protocols.io.81wgbx99qlpk/v1
External link: https://doi.org/10.1038/s41467-024-53168-7
Protocol Citation: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Novel Object Recognition Test. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbx99qlpk/v1
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 23, 2023
Last Modified: November 23, 2023
Protocol Integer ID: 91357
Keywords: mice, test
Abstract
Novel Object Recognition Test for mice
Troubleshooting
Individualize each mouse in a different cage with new bedding.
Expose the animal to two identical objects for habituation.
After 24h change one of the two objects for a new one.
Measure the time the animal spends exploring the new object and the old object.
Calculate the discrimination index using the formula: [time exploring the new object-time exploring the familiar object]/total time).