Sep 13, 2022

Public workspaceNoninvasive RNA-sequencing collection and processing

DOI
https://dx.doi.org/10.17504/protocols.io.kqdg3pjzzl25/v1
  • mem 1,2
  • 1Columbia University;
  • 2New York Genome Center
mem
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DOI: https://dx.doi.org/10.17504/protocols.io.kqdg3pjzzl25/v1
Collection Citation: mem 2022. Noninvasive RNA-sequencing collection and processing. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3pjzzl25/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
The results of this protocol are published and it is currently in active use.
Created: July 29, 2022
Last Modified: September 13, 2022
Collection Integer ID: 67892
Keywords: noninvasive rna, bulk rna extraction, performing bulk rna extraction, rna extraction, transcriptome study, collecting noninvasive sample, transcriptome, massive scaling of transcriptome study, sequencing collection, noninvasive sample, rna, sequencing, low cost, extraction, library preparation
Funders Acknowledgements:
Molly Martorella
Grant ID: 5 F30 HG011194
Abstract
This set of protocols accompanies the publication "Low cost, noninvasive RNA-sequencing to enable massive scaling of transcriptome studies" by Martorella et al.. Here you will find procedures for collecting noninvasive samples and performing bulk RNA extraction and library preparation using our low-cost method.
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Icon representing the file saliva_oragene_rna_extraction.pdf
saliva_oragene_rna_extraction.pdf
Icon representing the file noninvasive_sample_collection.pdf
noninvasive_sample_collection.pdf
Icon representing the file noninvasive_sample_rna_extractions.pdf
noninvasive_sample_rna_extractions.pdf
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noninvasive_sample_loseq_libprep.pdf