Jun 17, 2024
Nissl Counterstaining - Cresyl Violet
- Núria Peñuelas1,
- Ariadna Laguna1,
- Miquel Vila1
- 1VHIR-CIBERNED-ASAP
- Vilalab Public
- Nuria
External link: https://doi.org/10.1038/s41467-024-53168-7
Protocol Citation: Núria Peñuelas, Ariadna Laguna, Miquel Vila 2024. Nissl Counterstaining - Cresyl Violet. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl49wmrgo5/v1
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 17, 2024
Last Modified: June 17, 2024
Protocol Integer ID: 101941
Keywords: cresyl violet for histological section, cresyl violet nissl, cresyl violet, nissl counterstaining, histological section
Abstract
Nissl Counterstaining with Cresyl Violet for histological sections
Delipidation Step
Ethanol 95%, 5min
Chloroform 100%, 10min (under the hood)
Ethanol 95%, 15 sec (quick rinse)
Ethanol 95%, 2min
Ethanol 70%, 1min30sec
dH2O, 2min
Cresyl Violet staining
Cresyl Violet solution, 1min
dH2O, 15sec or less (quick rinse)
dH2O, 2min
Differentiation
Ethanol 70%+Acid Acetic*, few seconds (control differentiation yourself). (*add 2-3 drops
of acetic acid 100% in 100ml ethanol 70%).
Dehydratation
Ethanol 95%, 5-10sec (quick rinse)
Ethanol 100%, 1min
Xylene, 1min or more (under the hood) until mounting
Mount coverslip with DPX (under the hood)