Sep 29, 2024

Public workspaceNielsen and Ford (2024) - Reduced striatal M4-cholinergic signaling following dopamine loss differentially contributes to parkinsonian and L-DOPA-induced dyskinetic behaviors V.2

DOI
https://dx.doi.org/10.17504/protocols.io.dm6gp336jvzp/v2
  • Beatriz Nielsen1,
  • Christopher P Ford1
  • 1University of Colorado Anschutz Medical Campus
Beatriz E Nielsen
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DOI: https://dx.doi.org/10.17504/protocols.io.dm6gp336jvzp/v2
Collection Citation: Beatriz Nielsen, Christopher P Ford 2024. Nielsen and Ford (2024) - Reduced striatal M4-cholinergic signaling following dopamine loss differentially contributes to parkinsonian and L-DOPA-induced dyskinetic behaviors. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp336jvzp/v2Version created by Beatriz E Nielsen
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 30, 2024
Last Modified: September 29, 2024
Collection Integer ID: 104325
Keywords: ASAPCRN, cholinergic signaling, dopamine loss, following dopamine loss, induced dyskinetic behavior, reduced striatal m4, parkinsonian, striatal m4, dyskinetic behavior, dyskinetic behaviors this collection, dopa
Funders Acknowledgements:
NIH
Grant ID: R01-NS95809
NIH
Grant ID: R01-DA35821
Aligning Science Across Parkinson’s
Grant ID: ASAP-020529
Parkinson's Foundation
Grant ID: PF-PRF-839073
Abstract
This collection contains protocols detailing methods used in Nielsen and Ford (2024)-Reduced striatal M4-cholinergic signaling following dopamine loss differentially contributes to parkinsonian and L-DOPA-induced dyskinetic behaviors.

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Protocol
Name
Stereotaxic Surgery
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
Protocol
Name
Motor behavioral assessment
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Name
Immunohistochemistry
Version 1
, CU Anschutz
Kelsey BarcombCU Anschutz
Protocol
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6-OHDA mouse model of Parkinson's disease
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Levodopa-induced dyskinesia mouse model
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Electrophysiology and 2-photon imaging of Ca+2-transients
Version 2
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Ex vivo electrophysiology
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Name
Western Blot
Version 1
, University of Colorado Anschutz Medical Campus
Beatriz E NielsenUniversity of Colorado Anschutz Medical Campus
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Name
Acute Brain Slices
Version 1
, CU Anschutz
Kelsey BarcombCU Anschutz