Oct 01, 2021

Public workspaceNGM Agar

DOI
dx.doi.org/10.17504/protocols.io.zy7f7zn
  • 1Baylor College of Medicine
  • Adrien Assie: I am not the author of this protocol. This is a standard protocol for C. elegans maintenance available on wormbook.
Adrien Assie
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DOI: dx.doi.org/10.17504/protocols.io.zy7f7zn
External link: http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
Protocol Citation: Adrien Assie, Buck Samuel 2021. NGM Agar. protocols.io https://dx.doi.org/10.17504/protocols.io.zy7f7zn
Manuscript citation:
Brenner, S. (1974). Genetics 77, 71.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 10, 2019
Last Modified: October 01, 2021
Protocol Integer ID: 22271
Keywords: C. elegans, Growing media, Caenorhabditis , nematode
Disclaimer
This is a standard protocol for C. elegans maintenance available on wormbook.
Abstract
NGM agar recipe from wormbook
Start with Amount975 mL water

Amount3.0 g NaCl

Amount2.5 g Peptone

Amount17 g Agar

Autoclave with stir bar
Cool to Temperature55 °C (faster in water bath)

Then add the following while stirring on heat plate (DO NOT OVERHEAT)
Amount0.5 mL of Concentration1 Molarity (M) CaCl2 (sterile)



Amount1 mL of 5 mg/mL Cholesterol (dissolved in ethanol)

Amount1 mL of Concentration1 Molarity (M) MgSO4 (sterile)


Amount25 mL of Concentration1 Molarity (M) Potassium Phosphate Buffer, pH 6.0 (sterile)


Pour into petri dishes using sterile technique
  • 60 mm dishes = About Amount7 mL per plate
  • 100 mm dishes = About Amount20 mL per plate