Nov 21, 2025

Nextera XT at 0.2X On the Mantis V.4

DOI
https://dx.doi.org/10.17504/protocols.io.j8nlk57y1l5r/v4
  • Allen Institute for Brain Science1
  • 1Allen Institute
Allen Institute
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DOI: https://dx.doi.org/10.17504/protocols.io.j8nlk57y1l5r/v4
Protocol Citation: Allen Institute for Brain Science 2025. Nextera XT at 0.2X On the Mantis. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk57y1l5r/v4Version created by Allen Institute
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 21, 2025
Protocol  Integer ID: 233113
Keywords: Nextera XT, Nextera, Formulatrix Mantis, Mantis, nextera library, nextera xt, pcr, formulatrix mantis instrument, well pcr plate, mantis protocol, national institute of mental health
Abstract
Protocol to generate Nextera libraries using 0.2x reagents in a 96-well PCR plate using the Formulatrix Mantis instrument.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Attachments
Icon for HT_031_Mantis_Chip_Cleaning.docx
HT_031_Mantis_Chip_C...
31KB
Icon for HT_043_How_To_Clean_Hoods.docx
HT_043_How_To_Clean_...
17KB
Icon for MB0196_Nextera_XT_at_0.2X_on_the_Mantis.docx
MB0196_Nextera_XT_at...
2MB
Materials
1.1. Associated Library paper work
1.2. 96-well plate with normalized cDNA at 50 pg/µl (Stored at 4ºC)
1.3. Library plate labels
1.4. 5x Eppendorf twin.tec® 96-well PCR Plates (VWR 47744-106)
1.5. 4x Formulatrix molecular grade HV chips for Mantis instrument (MCHSVM96)
  1. Filtered pipette tips (GP-L200F, GP-L1000F)
1.7. Nextera XT DNA Sample Preparation Kit for 96 Samples (Illumina FC-131-1096)
1.7.1. Tagment DNA Buffer (TD) (15027866)
1.7.2. Amplicon Tagmentation Mix (ATM) (15031561)
1.7.3. Nextera PCR Master Mix (NPM) (15027920)
1.7.4. Neutralize Tagment Buffer (NT) (15031559)
1.8. Microseal ‘B’ Adhesive seal (BioRad MSB1001)
1.9. VWR Aluminum Foil seals for PCR, Sterile (VWR 60941-076)
1.10. Index primers combined at 2.5 µM each in 96 well plate format.
1.11. Small Kimwipes (VWR 21905-026)