Sep 03, 2019

Public workspaceNextera XT at 0.2X On the Mantis V.1

DOI
dx.doi.org/10.17504/protocols.io.63rhgm6
  • ZengU19 BRAIN Grant1
  • 1Allen Institute
  • BICCN / BICAN
  • Allen Institute for Brain Science
Dillan Brown
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DOI: dx.doi.org/10.17504/protocols.io.63rhgm6
Protocol Citation: ZengU19 BRAIN Grant 2019. Nextera XT at 0.2X On the Mantis. protocols.io https://dx.doi.org/10.17504/protocols.io.63rhgm6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 03, 2019
Last Modified: November 19, 2020
Protocol Integer ID: 27473
Keywords: Nextera XT, Nextera, Formulatrix Mantis, Mantis
Abstract
Protocol to generate Nextera libraries using 0.2x reagents in a 96-well PCR plate using the Formulatrix Mantis instrument.
Attachments
Icon for Nextera XT SOP.docx
Nextera XT SOP.docx
456KB
Materials
1.1. Associated Library paper work
1.2. 96-well plate with normalized cDNA at 50 pg/µl (Stored at 4ºC)
1.3. Library plate labels
1.4. 5x Eppendorf twin.tec® 96-well PCR Plates (VWR 47744-106)
1.5. 4x Formulatrix molecular grade HV chips for Mantis instrument (MCHSVM96)
  1. Filtered pipette tips (GP-L200F, GP-L1000F)
1.7. Nextera XT DNA Sample Preparation Kit for 96 Samples (Illumina FC-131-1096)
1.7.1. Tagment DNA Buffer (TD) (15027866)
1.7.2. Amplicon Tagmentation Mix (ATM) (15031561)
1.7.3. Nextera PCR Master Mix (NPM) (15027920)
1.7.4. Neutralize Tagment Buffer (NT) (15031559)
1.8. Microseal ‘B’ Adhesive seal (BioRad MSB1001)
1.9. VWR Aluminum Foil seals for PCR, Sterile (VWR 60941-076)
1.10. Index primers combined at 2.5 µM each in 96 well plate format.
1.11. Small Kimwipes (VWR 21905-026)