Aug 14, 2025

Public workspaceNeuromodulator Control of Energy Reserves in Dopaminergic Neurons

Proceedings of the National Academy of Sciences of the United States of America
DOI
https://dx.doi.org/10.17504/protocols.io.ewov11q42vr2/v1
  • Camila Pulido1,2,
  • Matthew Gentry3,
  • Tim Ryan1,2
  • 1Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland 20815, USA;
  • 3Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL
Camila Pulido
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DOI: https://dx.doi.org/10.17504/protocols.io.ewov11q42vr2/v1
External link: https://doi.org/10.1073/pnas.2523019122
Collection Citation: Camila Pulido, Matthew Gentry, Tim Ryan 2025. Neuromodulator Control of Energy Reserves in Dopaminergic Neurons. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov11q42vr2/v1
Manuscript citation:
Pulido C, Gentry MS, Ryan TA Neuromodulatory control of energy reserves in dopaminergic neurons. Proceedings of the National Academy of Sciences of the United States of America 122(50). doi: 10.1073/pnas.2523019122
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 14, 2025
Last Modified: August 14, 2025
Collection Integer ID: 224721
Keywords: ASAPCRN, Glycogen, Metabolism, Synapse, Dopaminergic Neurons, neuromodulator control of energy reserve, neuromodulator control, dopaminergic neurons the current collection, neuromodulator, dopaminergic neuron, control of energy reserve, energy reserve, control
Funders Acknowledgements:
ASAP
Grant ID: 000580
ASAP
Grant ID: 020608
Abstract
The current collection contains all experimental protocols associated with the study "Neuromodulator Control of Energy Reserves in Dopaminergic Neurons".
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Files
Protocol
Name
Primary Ventral Midbrain Neuronal Culture and Transfections
Version 1
, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
Protocol
Name
Primary hippocampal and cortical neuronal culture and transfection
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Name
Protocol for Neuronal Live-imaging of primary cultures
Version 1
, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
Protocol
Name
Semi-Automated Analysis Pipeline for Synaptic Function Studies Using iATPSnFR2-miRFP670nano3 biosensors
Version 1
, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
Protocol
Name
Semi-Automated Analysis Pipeline for Synaptic Function Studies Using pHluorin Biosensors
Version 1
, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
Protocol
Name
Immunofluorescence for adherent cells
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine