Nov 02, 2021

Public workspaceNeural activation of the GI tract

DOI
dx.doi.org/10.17504/protocols.io.bzp5p5q6
  • 1California Institute of Technology
  • Mazmanian Lab
Jessica Griffiths
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DOI: dx.doi.org/10.17504/protocols.io.bzp5p5q6
Protocol Citation: Bryan Yoo, Jessica Griffiths, Sarkis Mazmanian 2021. Neural activation of the GI tract. protocols.io https://dx.doi.org/10.17504/protocols.io.bzp5p5q6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: November 02, 2021
Last Modified: November 02, 2021
Protocol Integer ID: 54749
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-000375
National Institutes of Health
Grant ID: GM007616 and DK078938
Department of Defense
Grant ID: PD160030
Caltech Center for Environmental and Microbial Interactions (CEMI)
Grant ID: n/a
Heritage Medical Research Institute
Grant ID: n/a
Emerald Foundation
Grant ID: n/a
Aligning Science Across Parkinson's
Grant ID: ASAP-020495
Abstract
Protocol for neural activation of the GI tract used in Yoo et al 2021
TH-Cre and ChAT-Cre mice are used for these experiments.
“Activated” mice are infected with AAV-PHP.S:hSYN1-DIO-hM3Dq-mRuby2 and “Control Mice” are infected with AAV-PHP.S:hSYN1-DIO-mRuby2. This is to control for both AAV-PHP.S-mediated expression and the effects of Compound 21 dihydrochloride (C21) (HelloBio, Princeton, NJ-HB6124).
C21 was injected intraperitoneally at a dose of 3mg/kg in both activated and control mice. Mice for time course experiments were single-housed in sterile cages with autoclaved water following first C21 administration (C21 administered for 10 consecutive days). Injections of C21 we
Mice for time course experiments were single-housed in sterile cages with autoclaved water following first C21 administration (C21 administered for 10 consecutive days).Injections of C21 were administered at the same time every day (10AM).