Feb 07, 2024

Nest Building Test

 Forked from Nest Building Test
This protocol is a draft, published without a DOI.
  • 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 3Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 4Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 5Adrienne Helis Malvin Medical Research Foundation, New Orleans, LA 70130-2685, USA;
  • 6Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA;
  • 7Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;
  • 8Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA
Jacquelyn Haytayan
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Protocol Citation: daniel.dautan , Per Svenningsson, Valina L. Dawson, Ted Dawson, Hanseok Ko 2024. Nest Building Test. protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2024
Last Modified: September 23, 2024
Protocol  Integer ID: 94865
Keywords: ASAPCRN, behavior, fine motor skills, nigrostriatal sensorimotor function in mice, nigrostriatal sensorimotor function, nest building test, mice
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Used to assess nigrostriatal sensorimotor function in mice. Based on the Deacon 2006 protocol.
Materials
Cotton nestlets
Singly house mice in individual, clean cages.
Place a paper tissue folded into a 5x5 cm square.
Following 48h the bedding was score between 0 to 5 as follows:
0- The mice did not touch the paper square.
1- The mice unfolded the paper square but did not tear it up.
2- The mice unfolded and started to tear down <10% of the paper square.
3- The mice tear down the paper square <50%.
4- The mice tear down the paper square, but the bedding is not perfect.
5- Perfect bedding.
Protocol references
Deacon, Robert MJ. "Assessing nest building in mice." Nature protocols 1.3 (2006): 1117-1119.