May 08, 2024

Public workspaceNebuloni, F. & Do, Q. B. et al. (2024) A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy V.2

DOI
dx.doi.org/10.17504/protocols.io.36wgqjwwxvk5/v2
  • 1Osney Thermofluids Institute, Department of Engineering Science, University of Oxford, Osney Mead, Oxford OX2 0ES, United Kingdom;
  • 2The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.;
  • 3Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, United Kingdom;
  • 4Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, United Kingdom;
  • 5Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA
Cláudia C. Mendes
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DOI: dx.doi.org/10.17504/protocols.io.36wgqjwwxvk5/v2
Collection Citation: Federico Nebuloni, Quyen Do, Richard Wade-Martins 2024. Nebuloni, F. & Do, Q. B. et al. (2024) A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqjwwxvk5/v2Version created by Cláudia C. Mendes
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: May 11, 2023
Last Modified: May 08, 2024
Collection Integer ID: 99444
Funders Acknowledgements:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: Grant ID: ASAP-020370
Abstract
This collection contains six protocols detailing methods used in Nebuloni, F. & Do, Q. B.et al. (2024) A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy.
Files
Protocol
Icon representing the file Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs)
Name
ForkDifferentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
Name
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
Version 1
,
Quyen Do
Protocol
Icon representing the file Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway
Name
Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Automatic flow in fluid-walled dumbbells driven by Laplace pressure
Name
Automatic flow in fluid-walled dumbbells driven by Laplace pressure
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
Name
Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Immunostaining of corticostriatal culture on fluid-walled dumbbells
Name
Immunostaining of corticostriatal culture on fluid-walled dumbbells
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford