Jul 31, 2020
Version 1
NEBNext End Repair Module E6050 V.1
- 1New England Biolabs
- New England Biolabs (NEB)Tech. support phone: +1(800)632-7799 email: info@neb.com
Protocol Citation: Isabel Gautreau 2020. NEBNext End Repair Module E6050. protocols.io https://dx.doi.org/10.17504/protocols.io.k64czgw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 07, 2017
Last Modified: July 31, 2020
Protocol Integer ID: 9148
Materials
STEP MATERIALS
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
Protocol materials
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
Mix the following components in a sterile microfuge tube:
Mix the following components in a sterile microfuge tube:
Fragmented DNA: variable
NEBNext End Repair Reaction Buffer (10X): 10 μl
NEBNext End Repair Enzyme Mix: 5 μl
Sterile H20 for a final volume of 100 μl: variable
Total volume: 100 μl
NEBNext End Repair Enzyme MixNew England BiolabsCatalog #E6051
NEBNext End Repair Reaction Buffer:New England BiolabsCatalog #E6052
Incubate in a thermal cycler for 30 minutes at 20°C.
Purify DNA sample on one column.