May 12, 2023

NCEM Drop - Paramyxo confirmation, generation of RNP (TM-014)

DOI
https://dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
  • 1CSIRO ACDP
sandra.crameri
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DOI: https://dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop - Paramyxo confirmation, generation of RNP (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9dq8pg3e/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 12, 2023
Last Modified: May 12, 2023
Protocol  Integer ID: 81822
Keywords: paramyxo confirmation, generation of rnp, generate rnp, rnp, ncem drop, ncem
Abstract
Generate RNP to confirm Paramyxoviurs
Protocol materials
nano-WCatalog ##2018-5ML
Deoxycholic acid sodium saltMerck MilliporeSigma (Sigma-Aldrich)Catalog #D-6750
HEADER
SAN:
SPEC No:
OPERATOR:

Generation of RNP
Prepare 0.5 Mass / % volume Deoxycholic acid sodium saltMerck MilliporeSigma (Sigma-Aldrich)Catalog #D-6750 in TC Water.

Mix 1:1 with the sample for 00:05:00

5m
Conventional
11m
Adsorb 10 µL sample to grid 00:10:00 , inspect to ensure sample does not dry out.

10m
Drain excess sample from grid using filter paper, leave wet.
Stain nano-WCatalog ##2018-5ML 00:01:00

1m
Drain & dry using filter paper