May 12, 2023
Version 1
NCEM Drop - Inactivation of sample on grid (TM-014) V.1
- 1CSIRO
Protocol Citation: sandra.crameri 2023. NCEM Drop - Inactivation of sample on grid (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.8epv59mr6g1b/v1Version created by sandra.crameri
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 12, 2023
Last Modified: May 12, 2023
Protocol Integer ID: 81819
Keywords: ncem drop, inactivation of sample, ncem, inactivation, sample
Abstract
NCEM Drop - inactivation of samples on grid
Guidelines
This procedure is used for inactivation of 3Z agents, useful if the virus has low titire.
Protocol materials
Glutaraldehyde aqueous solution 25% EM Grade ampoulesProSciTechCatalog #C002
nano-WCatalog ##2018-5ML
Adsorb 10 µL sample to grid 00:10:00
10m
Immerse grid in drop of 2.5 % volume Glutaraldehyde aqueous solution 25% EM Grade ampoulesProSciTechCatalog #C002 in 0.1 Molarity (M) Sorenson's phosphate buffer 00:10:00
10m
Wash 0.1 Molarity (M) Sorenson's phosphate buffer
Using filter paper wick excess buffer from grid - do not dry out, leave grid surface wet.
Stain nano-WCatalog ##2018-5ML 00:01:00
1m
Using filter paper gradually wick stain dry from grid