May 13, 2023

NCEM Drop - Inactivation of sample in solution (TM-014) V.2

DOI
https://dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2
  • 1ACDP CSIRO
sandra.crameri
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2
Protocol Citation: sandra.crameri 2023. NCEM Drop - Inactivation of sample in solution (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2Version created by sandra.crameri
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 13, 2023
Last Modified: May 13, 2023
Protocol Integer ID: 81820
Keywords: proir to conventional ncem, conventional ncem, ncem drop, inactivation of sample, inactivation procedure, sample for biological transfer, ncem, inactivation, biological transfer, procedure, sample
Abstract
This inactivation procedure is used to inactivate a sample for Biological Transfer out of PC4, proir to Conventional NCEM.
Protocol materials
Paraformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004
Paraformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004
nano-WCatalog ##2018-5ML
Inactivation
10m
Add 50 µL 16 % volume Paraformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004 to a Sarstedt tube with o-ring (STK#:2056-U, Catalog #61226C2).

Add 150 µL sample to the Paraformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004 already in the tube. Leave contact time 00:10:00


10m
Adsorb 10 µL sample to grid 00:10:00 , inspect to ensure sample doesn't dry out.

10m
Drain excess sample from grid using fliter paper, leave wet.
Stain nano-WCatalog ##2018-5ML 00:01:00

1m
Drain & dry using filter paper