May 13, 2023

NCEM Drop Agar (TM-014)

DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
  • 1CSIRO ACDP
sandra.crameri
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop Agar (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzb4n5vx1/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 13, 2023
Last Modified: May 13, 2023
Protocol  Integer ID: 81826
Keywords: ncem drop, ncem
Abstract
drop agar
Protocol materials
nano-WCatalog ##2018-5ML
AgaroseCatalog #A5304
HEADER
SAN:
SPEC No:
OPERATOR:

Agar preparation
Make 2 Mass / % volume AgaroseCatalog #A5304 in TC Water. Microwave to dissolve, pour into3 mL of the solution into 5 mL petri dishes and place in fridge to solidify.

Cut 1 x1 cm squares out of agar with scalpel, lift out onto filter paper
Adsorption & Staining
1m
Place 5 µL sample on surface of agar square. Immediately float carbon coated formvar filmed grid face down on the droplet and leave until the droplet diffuses into the agar.

Stain nano-WCatalog ##2018-5ML 00:01:00

1m
Drain & dry using filter paper