Aug 26, 2022
Version 1
Nasal Lavage Sample collection and processing V.1
- maria.xylaki 1
- 1University Medical Center Goettingen
Protocol Citation: maria.xylaki 2022. Nasal Lavage Sample collection and processing . protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx9keog8j/v1
Manuscript citation:
Schoenebeck B, May C, Güldner C, Respondek G, Mollenhauer B, Hoeglinger G, et al. Improved preparation of nasal lavage fluid (NLF) as a noninvasive sample for proteomic biomarker discovery. Biochim Biophys Acta - Proteins Proteomics. Elsevier B.V.; 2015;1854:741–5.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 25, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 69180
Keywords: nasal, lavage, fluid, olfactory, sample, collection, ASAPCRN, nasal lavage fluid sampling, nasal lavage sample collection, proteome analysis, microbiome analysis, sample, omic
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Abstract
This protocol describes the nasal lavage fluid sampling that can be used for Omics (for Proteome analysis, see Schoenebeck et al, 2015; for Microbiome analysis see Heintz-Buschart et al, 2017) or RT-QuIC. Collection proceedure was adapted from B. Schoenebeck et al. 2015 (doi:10.1016/j.bbapap.2015.01.015).
Materials
- benchtop centrifuge with cooling function
- regular lab equipment (pipetts, tips, tubes)
- plastic disposable pipettes
- NaCl 0.9%, 10% and 20% (w/v) aqeous solutions
Troubleshooting
Nasal Lavage Sample collection
Two nasal lavage samples (NLS) are collected per person in each step, one from each nostril.
Both nasal cavities are pre-washed with 2 mL of physiological NaCl solution with the use of a plastic disposable pipette to remove bacterial impurities.
After 5 mins of incubation, each nasal cavity is rinsed again with 2 mL physiological NaCl solution with subsequent collection of the NLS into a tube.
Following this step, an additional rinse is performed with 2 mL NaCl 10% (w/v) aqeous solution and collection of the NLS into a tube.
A final rinse is performed with 2 mL NaCl 20% (w/v) aqeous solution and collection of the NLS into a tube.
Depending on downstream applications, NLS samples can be immediatelly frozen at -80 °C until analysis or centrifuged at 10.000 x g for 5 mins at 4 °C in order to remove cellullar debri and then stored.