License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
The opinions expressed here do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated. The protocol content here is under development and is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
This collection of protocols covers library preparation and sequencing on Oxford Nanopore platforms as well as bioinformatic analysis and submission of SARS-CoV-2 data. These protocols have been assembled by the Technical Outreach and Assistance for States Team (TOAST) and the Office of Advanced Molecular Detection (OAMD) at the Centers for Disease Control and Prevention for use by participating State, Tribal, Local and Territorial labs for sequencing of SARS-CoV-2. Individual protocols were developed by the protocol authors, and individual protocols should be credited accordingly.
These documents are intended for laboratories designated by TOAST and should not be widely or openly disseminated.
TOAST Nanopore protocol menu overview
For technical assistance, please contact: TOAST@cdc.gov
The opinions expressed here do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated. The protocol content here is under development and is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
