The Modified Ziehl-Neelsen stain (mZN stain)\u00a0is a type of differential bacteriological stain used to identify acid-fast organisms, mainly\u00a0Mycobacteria. Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid (fast=holding capacity). Members of the Actinomycetes, genus\u00a0Nocardia\u00a0(N.\u00a0brasiliensis\u00a0and N.\u00a0asteroides\u00a0are opportunistic pathogens) are partially acid-fast. Oocysts of coccidian parasites, such as\u00a0Cryptosporidium\u00a0and\u00a0Isospora, are also acid-fast. Hence they can also be detected and identified through mZN staining procedure.