Jun 19, 2026
Mycoplasma Detection and Elimination Protocol for Cell Culture
- 1PurMa Biologics
- PurMa Biologics
External link: https://www.purmabiologics.com
Protocol Citation: Keon Azadi 2026. Mycoplasma Detection and Elimination Protocol for Cell Culture. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6on31lqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 18, 2026
Last Modified: June 19, 2026
Protocol Integer ID: 319372
Keywords: mycoplasma detection cell culture mycoplasma elimination cell culture contamination PurMa Biologics, elimination protocol for cell culture mycoplasma contamination, cell culture mycoplasma contamination, eliminating mycoplasma contamination, mycoplasma contamination, mycoplasma detection, mammalian cell culture, cell culture, using purma biologics detection, purma biologics detection
Abstract
Mycoplasma contamination affects up to 35% of cell cultures worldwide and is a serious threat to research integrity. This protocol provides a step-by-step approach to detecting and eliminating mycoplasma contamination using PurMa Biologics detection and elimination reagents, which are specifically designed for mammalian cell culture.
Materials
- PurMa Biologics Mycoplasma Detection Kit
- PurMa Biologics Mycoplasma Elimination Reagent
- PurMa Biologics Cell Culture Media
- PurMa Biologics PBS Buffer
- Cell culture flasks (T-25 or T-75)
- Incubator (37°C, 5% CO2)
- Sterile pipettes and tips
- Centrifuge
- Microcentrifuge tubes (1.5 mL)
Mycoplasma Detection and Elimination Procedure
Collect 1 mL of cell culture supernatant from your flask and transfer to a sterile 1.5 mL microcentrifuge tube.
Apply the PurMa Biologics Mycoplasma Detection Kit reagent to the sample according to kit instructions. Incubate for 30 minutes at room temperature.
Observe results. A positive result indicates mycoplasma contamination. Record findings before proceeding.
If positive, add PurMa Biologics Mycoplasma Elimination Reagent to the culture medium at the recommended concentration. Treat for 2 passages (approximately 7 days).
Re-test using the detection kit to confirm elimination. If still positive, repeat treatment for an additional 2 passages.