Mar 18, 2024
MycoEd Lab: CU Denver Protocols
- Andrew Wilson1,
- sara.branco 2
- 1Denver Botanic Gardens;
- 2University of Colorado Denver
- MycoEd
Collection Citation: Andrew Wilson, sara.branco 2024. MycoEd Lab: CU Denver Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.261ge5pojg47/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 17, 2024
Last Modified: March 18, 2024
Collection Integer ID: 96830
Keywords: mycology, genomics, fungi, culturing, endophytes, mycology course, mycoed lab, harvest mycelium for genomic sequencing, fungi, fungi from plant, fall 2023 mycology course, harvest mycelium, cu denver protocols these lab protocol, department of integrative biology, online tools for gene assessment, culture assay, gene assessment, lab protocol, lab, using culture assay, university of colorado denver, class semester, class semester long project, cu denver protocol, additional exercises in bioinformatics, phylogenetic analysis, bioinformatics, integrative biology, genomic sequencing, plant, scientific research poster, dna for genotyping, dna, student
Abstract
These lab protocols were written for the Fall 2023 Mycology course at University of Colorado Denver, Department of Integrative Biology instructed by Sara Branco and Andrew Wilson. The course is designed to guide students through an in-class semester long project that isolates fungi from plants, assess phenotypic variation using culture assays, extract DNA for genotyping/identification, harvest mycelium for genomic sequencing. Additional exercises in bioinformatics use phylogenetic analysis and online tools for gene assessments in fungi. The course ends with the students producing a scientific research poster based on their results.
Troubleshooting
Files
MycoEdUCD_Lab1_Using_iNat.pdf
MycoEdUCD_Lab2_SampleAndCultureEndophytes2.pdf
MycoEdUCD_Lab3_IsolatingEndophytes.pdf
MycoEdUCD_Lab4_EndophyteBarcodingAndAssays.pdf
MycoEdUCD_Lab5_DNA_ExtractionAndPCR.pdf
MycoEdUCD_Lab6_GelElectrophoresis_SequencingPrep.pdf
MycoEdUCD_Lab7_SequenceEditingAndAnalysis.pdf
MycoEdUCD_Lab8_SequenceEditingAndAnalysis.pdf
MycoEdUCD_Lab10_Intro_To_Phylogenetics.pdf
MycoEdUCD_Lab9_Visit_Denver_Botanic_Gardens.pdf
MycoEdUCD_Lab11_Sexual_Reproduction_in_Ascomycetes.pdf
MycoEdUCD_Lab12_Fungal_Natural_Products_Production.pdf
MycoEdUCD_Lab13_DataHousekeeping.pdf
MycoEdUCD_Lab14_AssayDataAnalysis_HarvestingMycelium_Genbank.pdf
MycoEdUCD_Lab15_Making_A_Poster.pdf