This method is best used on colonies grown\u00a0on solid media and subcultures in 7H9 liquid media. This method is high yeilding and is very efficient for short read NGS. It is not appropriate for direct tissue or fecal extraction. It is also not appropriate for liquid media containing high amounts of egg yolk.\u00a0The use of trade, firm, or corporation names in this protocol is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture of any product or service to the exclusion of others that may be suitable.