May 22, 2025

Public workspaceMyco-Ed Protocols Booklet

DOI
dx.doi.org/10.17504/protocols.io.rm7vzqd5xvx1/v1
  • 1Department of Botany and Plant Pathology, Oregon State University;
  • 2Department of Research and Conservation, Denver Botanic Gardens;
  • 3Department of Biological Sciences, California State University Chico;
  • 4Department of Integrative Biology, University of Colorado Denver;
  • 5DOE Joint Genome Institute, Lawrence Berkeley National Laboratory;
  • 6Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory;
  • 7Department of Agricultural Biology, Colorado State University;
  • 8Department of Biology, Georgia Southern University;
  • 9School of Biology and Ecology, University of Maine;
  • 10Department of Biology, Tuskegee University;
  • 11Department of Ecology and Evolutionary Biology, University of Colorado Boulder;
  • 12Arizona Genomics Institute, University of Arizona;
  • 13Department of Plant and Microbial Biology, University of California Berkeley
  • MycoEd
Gerald Cobian
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DOI: dx.doi.org/10.17504/protocols.io.rm7vzqd5xvx1/v1
Document Citation: Amy Honan, Andrew W. Wilson, Gerald Cobian, sara.branco, Stephen J Mondo, Kerrie Barry, Sara Gremillion, Peter Avis, Jane Stewart, Ellen Dow, Chinyere Knight, Kurt LaButti, Alisha Quandt, Jayson Talag, Lotus Lofgren 2025. Myco-Ed Protocols Booklet. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzqd5xvx1/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: May 19, 2025
Last Modified: May 22, 2025
Document Integer ID: 218550
Keywords: mycology, genomics, fungi, culturing, endophytes, phenotype
Funders Acknowledgements:
JGI Director’s Science Program award
Grant ID: 509937
Abstract
This protocols booklet contains protocols designed to guide instructors and students through an in-class project that isolates fungi from plants, assess phenotypic variation using culture assays, extract DNA for genotyping/identification, harvest mycelium for genomic sequencing, and bioinformatics using phylogenetic analysis and online tools for gene assessments in fungi.
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