Jul 24, 2024

MSD + S + AS

This  protocol  is a draft, published without a DOI.
  • 1UGA
Eleanor Harman
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Protocol Citation: Eleanor Harman 2024. MSD + S + AS. protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 23, 2024
Last Modified: July 24, 2024
Protocol  Integer ID: 103947
Keywords: msd, a
Abstract
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Media
MS Media: 4.4g/L 2.2g/500mL
Sucrose: 30g/L 15g/500mL
Sorbitol(5%): 50g/L 25g/500mL
Thymidine: 300mg/L 150mg/500mL
2,4D: 2mg/L 1mg/500mL
Phytagel(1.4%): 14g/L 7g/500mL
Add 80% final volume DI water to beaker
Add MS Media, Sucrose, Sorbitol, Thymidine, and 2,4D until fully dissolved
Adjust pH to 5.8

Add DI water until final volume
Autoclave on 20 minute liquid cycle