Jul 25, 2024

Public workspaceMSD + S + AS

This protocol is a draft, published without a DOI.
  • 1UGA
Eleanor Harman
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Protocol Citation: Eleanor Harman 2024. MSD + S + AS. protocols.io https://protocols.io/view/msd-s-as-dhrj354n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 24, 2024
Last Modified: July 25, 2024
Protocol Integer ID: 103947
Abstract
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Media
Media
MS Media: 4.4g/L 2.2g/500mL
Sucrose: 30g/L 15g/500mL
Sorbitol(5%): 50g/L 25g/500mL
Thymidine: 300mg/L 150mg/500mL
2,4D: 2mg/L 1mg/500mL
Phytagel(1.4%): 14g/L 7g/500mL
Add 80% final volume DI water to beaker
Add MS Media, Sucrose, Sorbitol, Thymidine, and 2,4D until fully dissolved
Adjust pH to 5.8

Add DI water until final volume
Autoclave on 20 minute liquid cycle