Oct 24, 2019
Mowiol mounting media
- 1Boston University
Protocol Citation: Michael Economo 2019. Mowiol mounting media. protocols.io https://dx.doi.org/10.17504/protocols.io.8p6hvre
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 24, 2019
Last Modified: October 24, 2019
Protocol Integer ID: 29150
Keywords: histology, mounting media, embedding media
Abstract
This makes a low cost mounting media that preserves many fluorescent dyes very well. It is a non-hardening media.
This protocol first published in Cold Spring Harbor Protocols.
Materials
MATERIALS
GlycerolMerck MilliporeSigma (Sigma-Aldrich)Catalog #G5516
0.2 M Tris Buffer pH 8.5Merck MilliporeSigma (Sigma-Aldrich)Catalog #T1503-100G
Mowiol® 4-88Merck MilliporeSigma (Sigma-Aldrich)Catalog #81381-50G
1.Mix glycerol and Mowiol; dissolve with frequent agitation for 1 h at room temperature.
2.Add H2O. Stir for 1 h at room temperature.
3.Add Tris-Cl. Incubate for 2 h at 50°C with occasional stirring (e.g., for 2 min every 20 min).
4.Centrifuge at 5000g for 15 min.
5.Store 3-mL aliquots of the supernatant for up to 12 mo at -20°C.
Mowiol often does not dissolve completely.