Dec 14, 2023
Mouse Perfusion Protocol
- Michael Lee1
- 1University of Minnesota
Protocol Citation: Michael Lee 2023. Mouse Perfusion Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1nmo7gr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 64069
Keywords: Mouse Perfusion Protocol, 1x K+-free PBS, Perfusion, ASAPCRN, mouse perfusion protocol this protocol, mouse perfusion protocol, mouse perfusion procedure, mouse, protocol detail, protocol
Funders Acknowledgements:
ASAP
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Abstract
This protocol details the mouse perfusion procedure.
Attachments
452-958.docx
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For perfusion with 1x K+-free PBS:
Place mouse in isoflurane chamber until unresponsive to toe pinch stimuli.
Proceed to cut abdominal skin down both sides to expose the diaphragm.
Cut around diaphragm to expose the heart.
Cut right atria and insert perfusion needle into left ventricle with pump set to 5 rpm .
Perfuse 80 mL - 100 mL K+-free PBS until the liver is clear and liquid expelling from heart is completely clear.
Carefully remove brain and cut on mid-sagittal line using brain matrix.
Drop-fix left hemisphere in cold 4% paraformaldehyde for histological processing.
Sub-dissect right hemisphere for regions of interest.