Jun 09, 2026
Version 9
Mouse Cardiac Perfusion Fixation and Brain Collection V.9
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2026. Mouse Cardiac Perfusion Fixation and Brain Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv51bejl1b/v9Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 09, 2026
Last Modified: June 09, 2026
Protocol Integer ID: 318784
Keywords: AF0075, perfusion, brain, mouse, cardiac, mouse cardiac perfusion fixation, intracardiac perfusion fixation, procedures for intracardiac perfusion fixation, intracardiac perfusion, postnatal mice, brain removal, fixation, mice, brain collection this protocol, including anesthesia, brain collection,
Abstract
This protocol describes the procedures for intracardiac perfusion fixation of postnatal mice, including anesthesia, exsanguination, fixation, brain removal and post-fixation storage.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
