Dec 18, 2023
Motor behavioral evaluation of mice
- 1National Institute of Health
Protocol Citation: Tae-Un Han 2023. Motor behavioral evaluation of mice. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9p5kqg3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 18, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 92470
Keywords: ASAPCRN, Rotarod, Beam walk, Motor behavior
Funders Acknowledgements:
ASAP
Grant ID: ASAP-000458
Abstract
This is a general protocol to test motor coordination of mice used models of neurological disorder
Rotarod test
Rotarod test
On first day, mice are acclimated to standing on a non-rotating rod (Rotamex 5, Columbus Instrument) for three 00:01:00 trials with 00:10:00 interval.
11m
Three hours later, mice are acclimated to walking on the rod over three 00:01:30 trials at a speed of 4 rpm
1m 30s
On next day, latency of mice staying in the rotarod is recorded with accelerating speed of 4-40 rpm increasing by 1 rpm every 8.3 seconds for a maximum of 00:05:00 per trial.
5m
Repeat experiment for three trials daily for two days
Beam walk test
Beam walk test
5m
5m
Mice are trained to cross beams of 1~1.5m length of round beam. 24mm and 18mm diameter of beams are used.
Beam-crossing training is done for three times for two days
During the experimental day, its motor coordination will be measured by determining the number of slips, the latency required to cross the beam as the mouse traverses the beam.
For actual experiment, mice crosses both beams twice per trial for three trials win 00:05:00 resting periods in between trials
5m