May 27, 2025

Public workspaceMotility analysis of iPSC-derived microglia

DOI
https://dx.doi.org/10.17504/protocols.io.n92ldndy8v5b/v1
  • Lucas Blasco-Agell1,2,
  • Meritxell Pons-Espinal1,2,
  • Jara Montero-Muñoz1,2,
  • Antonella Consiglio1,2,
  • Miquel Vila3
  • 1Department of Pathology and Experimental Therapeutics, Bellvitge University Hospital- IDIBELL, 08908 Hospitalet de Llobregat, Spain;
  • 2Institute of Biomedicine of the University of Barcelona (IBUB), Carrer Baldiri Reixac 15-21, Barcelona 08028, Spain;
  • 3VHIR-CIBERNED-ASAP
  • Vilalab Public
Miquel Vila
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DOI: https://dx.doi.org/10.17504/protocols.io.n92ldndy8v5b/v1
Protocol Citation: Lucas Blasco-Agell, Meritxell Pons-Espinal, Jara Montero-Muñoz, Antonella Consiglio, Miquel Vila 2025. Motility analysis of iPSC-derived microglia. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ldndy8v5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 26, 2025
Last Modified: May 27, 2025
Protocol Integer ID: 218935
Keywords: microglia motility analysis of ipsc, derived microglia motility analysis, motility analysis of ipsc, motility analysis, ipsc, microglia
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Motility analysis of iPSC-derived microglia
Troubleshooting
Motility of iPSC-derived microglia (hMG) are assessed in 16 hours-time lapse experiments and analyzed using the Manual Tracker plug of ImageJ.
Analyses are done with Chemotaxis and Migration Tool free software version 2.0 (Ibidi‱). Velocity (μm/minute) and accumulated distance are evaluated. Accumulated distance refers to the path made by the cell from the starting point to the endpoint.