Jun 24, 2025

Molecular Cartography/Resolve OCT Sectioning V.2

DOI
https://dx.doi.org/10.17504/protocols.io.bp2l68bxrgqe/v2
  • 1Stanford University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
    Tech. support email: [email protected]
Joanna Bi
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DOI: https://dx.doi.org/10.17504/protocols.io.bp2l68bxrgqe/v2
Protocol Citation: Joanna Bi 2025. Molecular Cartography/Resolve OCT Sectioning. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l68bxrgqe/v2Version created by Joanna Bi
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 24, 2025
Last Modified: June 24, 2025
Protocol  Integer ID: 220938
Keywords: oct samples for molecular cartography, sectioning oct sample, molecular cartography, oct sectioning protocol, oct, resolve, resolve oct sectioning protocol
Abstract
Protocol for sectioning OCT samples for Molecular Cartography/Resolve.
Set cryostat to -20° C. Place samples inside to acclimate to temperatures for 10 minutes.
Add OCT media to the chuck and place the sample block on top. Let freeze for 5 minutes.
Insert and fasten cryostat blade and set sectioning thickness to 30-60 um.
Place chuck in specimen station and adjust sample distance and angle to blade.
Trim sample block until desired depth is reached.
Lightly score block with a razor blade so that tissue fits within the designated scan areas on the 8-sample Resolve slides.
Section at 10 um (or other desired thickness) and place section within the a scan area of the slide.
Repeat as needed to fill the slide.
Store slides at -80°C directly after sectioning.