This procedure is optimized for the isolation of 106 cells per test. If working with fewer than 106 cells, keep volumes as indicated for 106 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Product description and procedure summary: Target cells are positively selected or depleted by incubating the sample with the biotin anti‐mouse CX3CR1 antibody followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the CX3CR1+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.