Protocol Citation: Heitman Lab, Amy Gladfelter 2023. Modified Dixon’s Agar (Heitman Lab). protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7rp11vwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 03, 2018
Last Modified: June 01, 2023
Protocol Integer ID: 17455
Keywords: growth of malassezia species, malassezia species, malassezia, agar, heitman lab, recipe
Abstract
Media recipe for growth of Malassezia species.
Attachments
Malt extract . 36 g
Mycological peptone (Oxoid L40) 10 g
Dessicated ox bile (Oxoid) . 10 g
Agar 20 g
Tween 60 (heated before use) 10 mL
Glycerol, 50% 4 mL
dH2O 986 mL
Sterilize by autoclaving at 121 °C for 00:15:00 .
Cool to 50 °C .