Aug 09, 2024

Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits V.3

  • 1VHIR-CIBERNED-ASAP;
  • 2Vall d'Hebron Research Institute
  • Vilalab Public
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Collection CitationAriadna Laguna, Núria Peñuelas, Miquel Vila 2024. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2qq1ql1y/v3Version created by Miquel Vila
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 09, 2024
Last Modified: August 13, 2024
Collection  Integer ID: 105031
Keywords: human neuronal catecholaminergic pigmentation in rodents recapitulates age, modelling human neuronal catecholaminergic pigmentation, human neuronal catecholaminergic pigmentation in rodents recapitulate, human neuronal catecholaminergic pigmentation, neurodegenerative deficit, neurodegenerative deficits method, multisystem neurodegenerative deficit, multisystem neurodegenerative deficits method, rodents recapitulates age, rodents recapitulate
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-020505
The Michael J. Fox Foundation for Parkinson’s Research
Grant ID: MJFF-007184 and MJFF-001059
Ministry of Science and Innovation
Grant ID: PID2020-116339RB-I00
EU Joint Programme Neurodegenerative Disease Research
Grant ID: AC20/00121
La Caixa Bank Foundation
Grant ID: ID 100010434
Parkinson’s U.K
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Methods used in the manuscript entitled "Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits"
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
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Open field
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Habituation and dishabituation (olfaction test)
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Blood pressure
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Heart rate
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Passive avoidance (step-down test)
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Beam test
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Novel Object Recognition Test
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Immunoblot
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Quantification of p62-positive inclusions
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RNA and protein extraction from bulk dissections
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Gene expression analysis by quantitative Real-Time PCR (qPCR)
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cDNA synthesis
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Stereology-mediated cell count using StereoInvestigator
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Immunofluorescence on paraffin sections
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Intracellular neuromelanin quantification
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Rodent brain processing for histological analyses (update)
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Masson-Fontana Staining
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Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
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Optical densitometry of neuronal fibers
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Immunohistochemistry on free-floating cryosections
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Hematoxylin and Eosin (H&E) Staining
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Nissl Counterstaining - Cresyl Violet
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Survival Analysis
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Reproduction Analysis
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Transgenic mouse colony maintenance
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In situ Hybridization for Tyrosinase
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In vivo microdialysis for striatal DA release
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Brain Clarification for Neuromelanin visualisation by OPT
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Determination of free and protein-bound DA and NE, 5HT and Ach and their metabolites and oxidation products by UPLC-MS/MS method
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