Aug 09, 2024

Public workspaceModelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits V.3

  • 1VHIR-CIBERNED-ASAP;
  • 2Vall d'Hebron Research Institute
  • Vilalab Public
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Collection CitationAriadna Laguna, Núria Peñuelas, Miquel Vila 2024. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2qq1ql1y/v3Version created by Miquel Vila
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: June 04, 2024
Last Modified: August 13, 2024
Collection Integer ID: 105031
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-020505
The Michael J. Fox Foundation for Parkinson’s Research
Grant ID: MJFF-007184 and MJFF-001059
Ministry of Science and Innovation
Grant ID: PID2020-116339RB-I00
EU Joint Programme Neurodegenerative Disease Research
Grant ID: AC20/00121
La Caixa Bank Foundation
Grant ID: ID 100010434
Parkinson’s U.K
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Methods used in the manuscript entitled "Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits"
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Files
Protocol
Icon representing the file Open field
Name
Open field
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Habituation and dishabituation (olfaction test)
Name
Habituation and dishabituation (olfaction test)
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Grip strength test
Name
Grip strength test
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Blood pressure
Name
Blood pressure
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Polysomnography
Name
Polysomnography
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Intestinal transit time
Name
Intestinal transit time
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Heart rate
Name
Heart rate
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Magnetic resonance imaging (MRI)
Name
Magnetic resonance imaging (MRI)
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Tail Suspension Test
Name
Tail Suspension Test
Version 2
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Passive avoidance (step-down test)
Name
Passive avoidance (step-down test)
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Beam test
Name
Beam test
Version 1
, Weill Cornell Medicine
Jacquelyn HaytayanWeill Cornell Medicine
Protocol
Icon representing the file Novel Object Recognition Test
Name
Novel Object Recognition Test
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Immunoblot
Name
Immunoblot
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Quantification of p62-positive inclusions
Name
Quantification of p62-positive inclusions
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file RNA and protein extraction from bulk dissections
Name
RNA and protein extraction from bulk dissections
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file Gene expression analysis by quantitative Real-Time PCR (qPCR)
Name
Gene expression analysis by quantitative Real-Time PCR (qPCR)
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file cDNA synthesis
Name
cDNA synthesis
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file Stereology-mediated cell count using StereoInvestigator
Name
Stereology-mediated cell count using StereoInvestigator
Version 1
,
joan.compte
Protocol
Icon representing the file Immunofluorescence on paraffin sections
Name
Immunofluorescence on paraffin sections
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Intracellular neuromelanin quantification
Name
Intracellular neuromelanin quantification
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Rodent brain processing for histological analyses (update)
Name
Rodent brain processing for histological analyses (update)
Version 3
,
joan.compte
Protocol
Icon representing the file Masson-Fontana Staining
Name
Masson-Fontana Staining
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Name
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Optical densitometry of neuronal fibers
Name
Optical densitometry of neuronal fibers
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Immunohistochemistry on free-floating cryosections
Name
ForkImmunohistochemistry on free-floating cryosections
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Hematoxylin and Eosin (H&E) Staining
Name
Hematoxylin and Eosin (H&E) Staining
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Nissl Counterstaining - Cresyl Violet
Name
Nissl Counterstaining - Cresyl Violet
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Survival Analysis
Name
Survival Analysis
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Reproduction Analysis
Name
Reproduction Analysis
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Transgenic mouse colony maintenance
Name
Transgenic mouse colony maintenance
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file In situ Hybridization for Tyrosinase
Name
In situ Hybridization for Tyrosinase
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file In vivo microdialysis for striatal DA release
Name
In vivo microdialysis for striatal DA release
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Brain Clarification for Neuromelanin visualisation by OPT
Name
Brain Clarification for Neuromelanin visualisation by OPT
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Determination of free and protein-bound DA and NE, 5HT and Ach and their metabolites and oxidation products by UPLC-MS/MS method
Name
Determination of free and protein-bound DA and NE, 5HT and Ach and their metabolites and oxidation products by UPLC-MS/MS method
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP