Apr 08, 2026
Mitoguazone in vivo injections
- 1KU Leuven
Protocol Citation: Aisha Sati 2026. Mitoguazone in vivo injections. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ldeeo8v5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 27, 2026
Last Modified: April 08, 2026
Protocol Integer ID: 259759
Keywords: ASAPCRN, mitoguazone in vivo injection, mitoguazone in vivo, using mitoguazone, mitoguazone, vivo injection, vivo
Funders Acknowledgements:
Aligning Science Across Parkinson's (ASAP)
Grant ID: ASAP-000458
Abstract
This protocol details a treatment paradigm using mitoguazone in vivo
Materials
EDTA coated tubes for blood collection.
Syringes for weekly drug administration.
Scale (precision 0.1 g) to weight the pups.
Protocol materials
Mitoguazone,Sanbio BVCatalog #HY-106634/CS-0026193
Before start
Working solution should be diluted 1:10 (=0.5 mg/ml)
Protocol
Mice are injected with 2 mg/kg Mitoguazone,Sanbio BVCatalog #HY-106634/CS-0026193 via subcutaneous injections at 1, 2, 3, 4, 5, 6, 7 and 8 weeks of age.
From 1 week of age, body weight of mice is monitored twice a week until 4 weeks of age, and once a week thereafter.
Drinking water bottles are weighed weekly.
At 2, 6 and 10 weeks of age, mouse blood is collected via the submandibular vein in
Equipment
EDTA coated tubes
NAME
Sarstedt
BRAND
201288
SKU
At 10 weeks of age, limb clasping, pole and open field tests are performed to assess the effect of the treatment on behavioral outcomes.