Oct 04, 2023
mitochodnrial assays in human muscle biopsies
- 1Wake Forest University School of Medicine
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Bumsoo Ahn 2023. mitochodnrial assays in human muscle biopsies. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxrb1lpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 19, 2023
Last Modified: October 04, 2023
Protocol Integer ID: 88033
Keywords: mitochodnrial assays in human muscle biopsy, mitochondrial sensitivity, mitochondrial respiration, mitochodnrial assay, human muscle biopsy tissue, human muscle biopsy
Disclaimer
Ahn Lab, Wake Forest University
Abstract
This protocol simultaneously measures mitochondrial respiration and hydrogen peroxide generation rates in human muscle biopsy tissues. The protocol also includes methods for measurement of mitochondrial sensitivity to ADP and responses to metformin.
Attachments
