Sep 02, 2024
Microplastics extraction from oyster tissue
- Benjamin-Rafael Mingoa1,
- Archana Anand1
- 1San Francisco State University
- Anand Lab
Protocol Citation: Benjamin-Rafael Mingoa, Archana Anand 2024. Microplastics extraction from oyster tissue . protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk81dxl5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 31, 2024
Last Modified: September 02, 2024
Protocol Integer ID: 106797
Keywords: Oyster, Microplastics, Tissue, Olympia oyster, microplastics extraction from oyster tissue, microplastics in oyster tissue, microplastics extraction, microplastic, microplastic particle, oyster tissue, size of microplastic, microplastic particles every time, plastic particle, such as detergent, detergent, many water filtration system, such as the san francisco bay, tissue, extraction, such as acrylic, nylon, polyester, acrylic
Funders Acknowledgements:
College of Science & Engineering, San Francisco State University
Abstract
Microplastics are defined as any plastic particles that are smaller than 5mm. They have been found in many of our household products, such as detergents, toothpaste, and other beauty products such as exfoliants. It has also been found that clothes with synthesized fibers, such as acrylics, nylon, and polyester shed hundreds of thousands of microplastic particles every time you wear or wash them. Due to the size of microplastics, they easily pass through many water filtration systems and end up in bodies of water, such as the San Francisco Bay or the Pacific Ocean. This protocol describes the procedure to extract and quantify microplastics in oyster tissue.
Preparation and extraction of microplastics from Oyster Gastrointestinal Tract/Tissues
Take samples of the gastrointestinal tract (GIT) /biofilm and gill tissues (fish gills have the same biofilm membrane)
Weigh out 1-2 g of the tissues. Use a glass cover for weighing.
Cut the sample with forceps to increase surface area
10% KOH, 50 mL,
(as a guideline 1:50 ratio of tissue to KOH works well)
incubate at 60 C for 24 hours
Filter through coarse mesh
Set up a filtration system (Whatman 1 filter, flask)
Soak the Whatman 1 filter in distilled water to prevent it from floating
USE VACUUM (or samples will be compromised due to floating)
Pour the KOH and tissue solution into the Whatman 1 filter. Vacuum.
Rinse sides of filter with distilled water
Carefully take the filter and place it into a glass plate with forceps
Wash with 50 mL boiling water
Incubate for 10 min with 10 mL acetone
Wash with another 10 mL of acetone
Staining with 1mL of diluted Nile Red
Rinse with 10 mL of water (wash the sides of the filter)
Place into a Petri plate
Observe, use a fluorescent scope