Oct 12, 2021
Version 4
Microfluidics 5: PDMS Microchannel Bonding on Glass/PDMS V.4
- Serhat Sevli1,
- C. Yunus Sahan1
- 1Nehir Biyoteknoloji Ltd. www.nehirbt.com
- NehirBTTech. support email: [email protected]
Protocol Citation: Serhat Sevli, C. Yunus Sahan 2021. Microfluidics 5: PDMS Microchannel Bonding on Glass/PDMS. protocols.io https://dx.doi.org/10.17504/protocols.io.byzepx3eVersion created by Serhat S
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: October 12, 2021
Last Modified: October 12, 2021
Protocol Integer ID: 54022
Keywords: microfluidics, PDMS, oxygen plasma, pdms microchannel bonding on glass, pdms microchannel bonding, pdms microfluidic chip, microfluidic chip, microfluidic, covalent bonding of pdm, pdm, liquid seal, complete covalent bonding between layer, covalent bonding, layer of glass, glass by air plasma technique, complete covalent bonding, glass
Abstract
Microfluidic chips, made of PDMS, are one-side open when fabricated. Another layer of glass, PDMS, or etc is needed. Liquid seal is provided by complete covalent bonding between layers or by external forces like threads, magnets or similar factors. This protocol describes the covalent bonding of PDMS on glass by air plasma technique.
Materials
Glass slide; Standard sizes microscope glass preferably Marienfeld Microscope Slides 1000000
PDMS material; Dow Corning, Sylgard 184
Troubleshooting
Safety warnings
* All the related steps must be done in a clean room classD (minimum) with appropriate clothing.
* All oxygen plasma generation procedures must be performed carefully and not alone.
* Please learn well about the hazards of plasma generators and PDMS material.
Before start
This protocol is derived from "soft lithography" literature and can be modified by user freely.
Oxygen Plasma Exposure
18m
1.0.1. Cured PDMS manufactured at previous steps using lithography or 3D printed molds are cut or removed from the mold and put inside a clean petri dish. Since PDMS is vulnerable to surface adsorption of dust, each must be clean and performed inside cleanroom facilities.
1.0.2. NehirBT's air plasma generator is a homemade setup of microwave instrument in which there is a glass vacuum desiccator.
Safety information
There are standard oxygen plasma devices commercially available. We are using a homemade air plasma generator modified from a microwave oven which is modified by including a desiccator inside and opening appropriate holes for vacuum tubing. Also in order to form a vacuum inside the desiccator, appropriate tubing is connected with the vacuum pump.
Please, do not try to make a similar modification yourself if you are not an expert on electronic devices.
Safety information
Do not use any kind of metal inside the microwave oven when it is on or off.
The purple plasma waves inside the instrument must not touch PDMS otherwise PDMS burns and bonding is not possible.
Safety information
The duration of plasma exposure depends on the power of plasma, the volume of the desiccator, the size of the PDMS film, and some others. So an optimization step is required for all different setups. If the duration of plasma exposure is too low the surfaces of PDMS and glass will not be cleaned enough to be bonded. On the other hand, if the plasma exposure time is long, the surface of PDMS will be scorched and will not bond on the glass again.
Equipment
Oxygen/Air Plasma
NAME
custom-made
BRAND
microwave incl. glass vacuum chamber
SKU
3m
1.1.1. PDMS material is cleaned inside a fume hood by isopropanol and dried by nitrogen gas spraying.
1.1.2. Glass slide is cleaned inside a fume hood by isopropanol and dried by nitrogen gas spraying.
5m
1.2.1. PDMS microchannels can be bonded on a glass microscope slide, glass petri dish, or another PDMS layer according to the necessities of the application.
1.2.2. The glass and PDMS materials are placed inside the desiccator inside the plasma generator system without touching each other. Glass petri dishes and/or glass cell flasks can also be used for PDMS bonding when their sizes fit inside the desiccator.
1.2.3. The lid of the desiccator is closed. The door of the instrument is closed well.
1.2.4. The vacuum pump is run for 5-6 minutes to remove the air inside the desiccator and to secure a low vacuum level.
1.2.5. Then the microwave is kept turned on till purple oxygen plasma waves are generated and observed. It is kept on for 5-6 seconds more with plasma generated inside the desiccator.
10m
Bonding After Exposure
2m
2.0.1. After exposure bonding and alignment is critical and a few minutes is present to keep plasma effect on materials.
2.1.1. When the alignment is important between layers, a special jig may be used. In this case, place the jig between layers perform the further steps.
1m
2.2.1. The plasma exposed PDMS layer is put on the other plasma exposed layer which may be glass or PDMS layer and both will bond permanently with covalent bonding.
2.2.2. The exposed PDMS and bottom layer may not be enforced for bonding, this behavior may cause over bonding of microchannels inside.
2.2.3. After bonding if there is any air present between layers, a plastic tip may be used to remove air by manual smooth pressure.
2.2.4. The duration of bonding may take a few minutes at room temperature.
1m
Final Heating and Ready
10m
3.0.1. The final PDMS on Glass/PDMS chip is heated at 85oC for 5-10 minutes on a heating plate or for 10 minutes in a heater oven.
Safety information
Do not forget to cool down the final chip product before touching and using it.
This step is performed inside the cleanroom.
Equipment
Hot Plate
NAME
Electromag
BRAND
LB.EM.M4060
SKU
10m
Stock of PDMS chips
3m
4.0.1. The prepared chips can be stored at room temperature inside a closed clean box or petri dish after sealing the top and down of channels with scotch tape and they can be used anytime.
Safety information
If the PDMS chips are left uncovered, dust on air will stick on the chip very soon.
3m
Visual Quality Check
1m
5.0.1. Visual check of bonding PDMS on glass layer is performed under a digital microscope, and these parameters are checked mainly;
5.0.2. Poor adhesion or poor bonding, Debris (Trace), air bubbles (active areas), Stains-Voids, Scratch marks, Broken PDMS, Uneven PDMS, Wrong Orientation (PDMS), Crooked trace, Chipped or Broken Glass, and Wrong Alignment of PDMS on Glass.
1m