Jun 15, 2024

Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments

Science Advances
DOI
https://dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
  • 1University of Minnesota
Philippa R Kennedy
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DOI: https://dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
External link: https://doi.org/10.1126/sciadv.adn1849
Collection Citation: Philippa Kennedy 2024. Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo3y9xv4o/v1
Manuscript citation:
Kennedy PR, Arvindam US, Phung SK, Ettestad B, Feng X, Li Y, Kile QM, Hinderlie P, Khaw M, Huang R, Kaufman M, Puchalska P, Russell A, Butler J, Abbott L, McClure P, Luo X, Lu QT, Blazar BR, Crawford PA, Lim J, Miller JS, Felices M Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments. Science Advances 10(44). doi: 10.1126/sciadv.adn1849
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 11, 2023
Last Modified: June 15, 2024
Collection  Integer ID: 84864
Keywords: function of therapeutic nk cell, therapeutic nk cell, hypoxic tumor environment, metabolic program, hypoxic tumor
Funders Acknowledgements:
Department of Defense
Grant ID: CA200922
Emily Whitehead Foundation
Grant ID: Nicole Gularte Fight for Cures
Abstract
A collection of protocols associated with the publication 'Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments' by Kennedy et al.
Files
Protocol
Name
Degranulation and cytokine production (functional assay)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Isolation of natural killer (NK) cells from human blood products
Version 2
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays)
Name
Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Expansion of NK cells on feeder cells
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Quantification of synapse polarization ("the conjugate assay")
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Proliferation assay
Name
Proliferation assay
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Mitochondrial staining of NK cells by flow cytometry
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Time of flight mass cytometry (CyTOF)
Name
Time of flight mass cytometry (CyTOF)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Adhesion assay
Name
Adhesion assay
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Cell line information
Version 4
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Time-lapse killing assay (monolayer - IncuCyte)
Name
Time-lapse killing assay (monolayer - IncuCyte)
Version 2
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Confocal microscopy of intracellular components within NK cells
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Impedance (xCELLigence)
Name
Impedance (xCELLigence)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Assessing IL-15 bioavailability ("the bioassay")
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota