Nov 21, 2020

Public workspaceMeasuring Fungal Growth

DOI
https://dx.doi.org/10.17504/protocols.io.bnu2meye
Measuring Fungal Growth
  • João raújo1
  • 1University of Florida
  • Protocols Bark Beetle Mycobiome
Bark Beetle Mycobiome Research Coordination Network Bark
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DOI: https://dx.doi.org/10.17504/protocols.io.bnu2meye
Document Citation: João raújo 2020. Measuring Fungal Growth. protocols.io https://dx.doi.org/10.17504/protocols.io.bnu2meye
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: October 23, 2020
Last Modified: November 21, 2020
Document Integer ID: 43642
Keywords: measuring fungal growth, bark beetle mycobiome, part of the bark beetle mycobiome, fungal growth this protocol, fungal growth, fungus symbiosis, fungus, widespread insect, more information on the bbm, symbiosis
Abstract
This protocol describes how to measure fungal growth.

This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F, Seltmann KC, Six D, Vanderpool D, & Villari C. 2020. Bark beetle mycobiome: collaboratively defined research priorities on a widespread insect-fungus symbiosis. Symbiosis 81: 101–113 https://doi.org/10.1007/s13199-020-00686-9.
Troubleshooting
  1. Petri dish with the culture you want to measure;
  2. Stereomicroscope with camera attached and software to make measurements;

Measuring Diameter
Carefully place the Petri dish of the fungal culture you want to measure on the stereomicroscope stage. Center it and adjust the focus. Select the measurement tool > straight line.
Draw a line from one side of the culture to another and record the length. 

Measuring area
Carefully place the Petri dish of the fungal culture you want to measure on the stereomicroscope stage. Center it and adjust the focus. Select measure tool > area. Carefully draw a line around the fungal culture, respecting the fungal culture edges and record the length.
Both can be done every day, every other day, once a week or according to each specific need.