The authors describe the skin thickness measurement, based on the analysis of images, obtained using a handheld DermaLab® USB Series ultrasound from Cortex Technology. Epidermis and dermis layers of newborns are obtained by ultrasound scanning. The procedures of the mean thickness determination are pictured.We developed a dedicated software to provide epidermal thickness measurement (IP register BR 51 2017 000061 1). The calculation of the epidermal thickness for each line in the image array was based on the size of the pixel in the frame obtaining 356 thickness values. Using a bootstrap technique in the selected sample size with 70 lines, the software resampled the thickness values 2,000 times. From a Gaussian distribution adjustment curve to the epidermis measurement, the peak was the thickness value, and the full with a half-maximum area was the sigma error value.