Feb 04, 2025
Measurement of protein aggregation with PROTEOSTAT
- Maria Jose Perez J.1
- 1Institut Imagine
- Team Deleidi
Protocol Citation: Maria Jose Perez J. 2025. Measurement of protein aggregation with PROTEOSTAT. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly6pymgx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 27, 2024
Last Modified: February 04, 2025
Protocol Integer ID: 112823
Keywords: protein aggregation with proteostat measurement, protein aggregation with proteostat, protein aggregation, proteostat measurement, protein, proteostat
Funders Acknowledgements:
ASAP
Abstract
Measurement of protein aggregation with PROTEOSTAT
Troubleshooting
Measurement of protein aggregation with PROTEOSTAT
Prepare the PROTEOSTAT‱ detection dye according to the manufacturer's instructions (ENZ-51023-KP050, Enzo Life Sciences).
Dilute the detection dye 1:2 in 1× assay buffer (provided in the kit).
Load 2 µL of the diluted detection dye into a 96-well plate with black walls and a clear bottom.
Load 30 µg of total protein for each sample into the same plate and dilute in assay buffer to a final volume of 100 µL if needed.
Incubate the plate for 15 minutes at room temperature in the dark.
Measure fluorescence at 550 nm excitation and 600 nm emission using a fluorescence plate reader (PerkinElmer Envision 2105).