Jan 14, 2020
Version 1
MCM Chromatin flow cytometry for cell cycle V.1
- Jacob Matson1,
- Jean Cook1
- 1University of North Carolina at Chapel Hill
External link: https://elifesciences.org/articles/30473
Protocol Citation: Jacob Matson, Jean Cook 2020. MCM Chromatin flow cytometry for cell cycle. protocols.io https://dx.doi.org/10.17504/protocols.io.bba8iihw
Manuscript citation:
1. Matson, J. P., House, A. M., Grant, G. D., Wu, H., Perez, J., and Cook, J. G. (2019) Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence. Journal of Cell Biology. 218(7): 2169-2184. Link: https://doi.org/10.1083/jcb.201902143 2. Matson, J. P., Dumitru, R., Coryell, P., Baxley, R. M., Chen, W., Twaroski, K., Webber, B. R., Tolar, J., Bielinsky, A.-K., Purvis, J. E., and Cook, J. G. (2017) Rapid DNA replication origin licensing protects stem cell pluripotency. eLife 6, e30473. Link: https://doi.org/10.7554/eLife.30473
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 14, 2020
Last Modified: January 14, 2020
Protocol Integer ID: 31808
Keywords: Chromatin flow cytometry, MCM, origin licensing, CDT1, cell cycle
Abstract
Chromatin flow cytometry for MCM loading cell cycle analysis, adaptable to any antibody measurements by chromatin flow cytometry.
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