Sep 25, 2024
Mass Spectrometry Identification of Samples Separated by Liquid Chromatography
- Caroline Green1
- 1Creative BioMart
- Creative BioMart
Protocol Citation: Caroline Green 2024. Mass Spectrometry Identification of Samples Separated by Liquid Chromatography. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l625kdgqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 25, 2024
Last Modified: September 25, 2024
Protocol Integer ID: 108296
Keywords: Mass Spectrometry Identification of Gel ..., Mass Spectrometric Identification of Phosphorylated Peptides and Phosphorylated Sites, Guide for Mass Spectrometry Identification of Samples Separated by Liquid Chromatography, liquid chromatography, mass spectrometry identification of sample, mass spectrometry identification, chromatography, fundamental chromatography theory, terms of fundamental chromatography theory, complex sample
Abstract
As one of the most powerful separation and analysis technologies in the field of chemistry, liquid chromatography (LC) has developed rapidly since the 1970s. Both in terms of fundamental chromatography theory and instrument performance, it has been greatly improved and improved. LC exhibits high separation efficiency and accurate quantification in the analysis of complex samples.
Troubleshooting
Guide for Mass Spectrometry Identification of Samples Separated by Liquid Chromatography
Main Instruments and Equipment
Material
Main reagents*1
Experimental Methods
Sample purification. Wash the protein with acetone pre-cooled at -20°C to remove impurities.
Trypsin digestion of the sample*2
Liquid chromatography column flushing and equilibration
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