Dec 08, 2021
Making worm picks
- 1Imperial College London
- Behavioural Genomics
Protocol Citation: Bonnie Evans 2021. Making worm picks. protocols.io https://dx.doi.org/10.17504/protocols.io.b2p3qdqn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: December 08, 2021
Last Modified: December 08, 2021
Protocol Integer ID: 55771
Abstract
Making platinum wire picks for transferring C.elegans onto plates
Put on safety glasses.
Use pliers to cut an appropriate length of platinum wire.
Light the ethanol burner. Take care that there is nothing directly above it, eg. papers attached to shelf.
Hold a disposable glass Pasteur pipette in one hand and the platinum wire with forceps in the other hand.
Place the platinum wire inside the pipette and move it above the flame for a short while to seal the glass around the wire. If preferred, use flat-ended pliers to flatten the end of the platinum wire.
Remove the pick from the flame and wait for it to cool down before touching.
Use the cap to extinguish the flame and check to see that it is gone out.