Mar 30, 2026
Making Kanamycin Base Stock Solution and Dilutions
Forked from How to make a 50 mg/ml Kanamycin Stock Solution
- Hendrick Read1
- 1UA iGEM
External link: http://store.p212121.com/kanamycin-monosulfate/
Protocol Citation: Hendrick Read 2026. Making Kanamycin Base Stock Solution and Dilutions. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk12z5g5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: February 18, 2026
Last Modified: March 30, 2026
Protocol Integer ID: 243601
Keywords: ml kanamycin stock solution, stock solution, ml, mg, kanamycin base stock solution, dilution
Abstract
Materials
MATERIALS
Kanamycin MonosulfateP212121Catalog #TWA-K-120
STEP MATERIALS
Kanamycin MonosulfateP212121Catalog #TWA-K-120
Kanamycin MonosulfateP212121Catalog #TWA-K-120
Creating Base Stock
Add 10 ml of sterile H2O.
Dissolve completely.
00:02:00
Pre-wet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water.
Sterilize Kanamycin Stock through the prepared 0.2 µm syringe filter.
Stock may be kept at -20°C for 1 year.
00:02:00
Diluting For Immediate Use (working concentration is 100µg/mL)
Dilution is to be done at 1:500 stock to water.
Ex: Mix 2 µL stock of the prepared stock with 998 µL water for a 1mL solution