Please contact Dr. Steven Wilhelm (email@example.com) for additional information regarding this protocol.Adapted from Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. S\u00f4rui no bunri to baiy\u00f4h\u00f4. 2.5.B. Tansui s\u00f4rui]. In Methods in Phycological Studies [S\u00f4rui Kenky\u00fbh\u00f4], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ).