Apr 28, 2020

Public workspaceLysis Buffer

DOI
dx.doi.org/10.17504/protocols.io.beefjbbn
  • 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Elisa Storelli
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DOI: dx.doi.org/10.17504/protocols.io.beefjbbn
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. Lysis Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.beefjbbn
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: March 31, 2020
Last Modified: June 09, 2020
Document Integer ID: 34983



Protocol
Separation and purification of human PBMC from BUFFY COAT
NAME

Separation and purification of human PBMC from BUFFY COAT

CREATED BY
Elisa Storelli


Lysis Buffer composition g/l (M)

NH4Cl 8.248 (0.154)

KHCO3 1.0 (0.01)

EDTA 0.0368 (0.0001)

Add ultrapure water to 1 liter.

Storage: Temperature4 °C Fridge 1 - (Room TSO8).

Filtered Lysis Buffer: filter lysis buffer using sterile syringe and filter.

Sterile filter and syringe are in closet 1 (Room PSO3).

Prymo siringe filter: catalog number EPSPE2230 Euroclone, Italy.

Storage: Temperature4 °C Fridge 2 - (Room PSO3).




NH4Cl: catalog number 1.01145.1000 Merck, Italy.

KHCO3:catalog number 1.04854.500 Merck, Italy.

EDTA: catalog number ED2SS Sigma, Italy.