Feb 17, 2017

Public workspaceLysis Buffer (20 mL)

DOI
https://dx.doi.org/10.17504/protocols.io.c4gytv
  • Matt Sullivan Lab1
  • 1Matt Sullivan Lab
  • VERVE Net
  • Sullivan Lab
Celina G Gomez
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DOI: https://dx.doi.org/10.17504/protocols.io.c4gytv
Protocol Citation: Matt Sullivan Lab 2017. Lysis Buffer (20 mL). protocols.io https://dx.doi.org/10.17504/protocols.io.c4gytv
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: June 18, 2015
Last Modified: January 31, 2018
Protocol Integer ID: 872
Keywords: lysis buffer, fresh before experiment, sucrose, experiment, buffer
Abstract
Must be made fresh before experiment because of the Sucrose.
For 20 mL solutions.
Guidelines
Final Concentration of Lysis Buffer
40 mM EDTA
50 mM Tris (pH 8.3)
0.73 M Sucrose
Materials
STEP MATERIALS
ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
ReagentTrisP212121Catalog #RP-T60040
ReagentSucroseP212121
ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
ReagentTrisP212121Catalog #RP-T60040
ReagentSucroseP212121
Protocol materials
ReagentSucroseP212121
ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
ReagentTrisP212121Catalog #RP-T60040
Troubleshooting
Before start
Prepare solution with sterile milliQ water and filter sterilize or autoclave before use.
1.6 mL of 0.5 M EDTA
ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
1.0 mL of 1 M Tris (pH 8.3)
ReagentTrisP212121Catalog #RP-T60040
5.13 g Sucrose
ReagentSucroseP212121