Jul 30, 2025
Version 1
LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model V.1
- Miquel Vila1
- 1VHIR-CIBERNED-ASAP
- Vilalab Public
Collection Citation: Miquel Vila 2025. LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7n8e9lwz/v1Version created by Miquel Vila
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 30, 2025
Last Modified: July 30, 2025
Collection Integer ID: 223653
Keywords: ipscbased parkinson, dopaminergic neurodegeneration, mutant microglia, neuromelanin, article lrrk2, lrrk2
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Collection of protocols included in article LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model
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Protocol
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iPSC derived microglia (hMG) culture
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iPSC-derived vmDAn
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iPSC-derived organoid generation
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2D and 3D Neuron/Microglia co-culture systems
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Immunocytochemistry
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Flow Cytometry
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Gene expression analysis
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Cytokine Array
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Synaptosome extraction
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Western Blot
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pHrodo labeled synaptosome phagocytic assay
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Neuromelanin phagocytosis assay
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Motility analysis of iPSC-derived microglia
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Intracellular ROS measurement in iPSC-derived microglia
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IBA1 Immunochemistry on human paraffin sections
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Intracellular neuromelanin quantification
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Isolation of pure neuromelanin extracts from cultured TR5TY6 neuroblastoma cells
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Preparation of α-synuclein pre-formed fibrils (PFF)
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